Chlorophyll Extraction

I tried another experiment last night. I had heard somewhere that if you boil green leaves, you can extract the chlorophyll from them when can then be excited by exposure to light, making the solution ‘glow’ as electrons move around. So I dutifully went out, gathered several leaves and put them in a beaker, which I heated over my Bunsen burner.

Note to self: Get new Bunsen Burner. After heating it for about 20 minutes and it still hadn’t boiled, I decided a new tactic was necessary.

Note to self: Buy more Denatured Alcohol.

I poured some of the liquid into a test tube, and added some of the leaves. Now the water started boiling pretty quickly. The water wasn’t getting any greener, though. After observing it boil for a while, I decided it was as good as it was going to get, and cut the flame. Then I removed the leaves, and exposed the final solution to a light source.

I was underwhelmed, to say the least.

There are several possibilities I have thought of as to what went wrong:

  1. Boiling leaves is not an efficient method of chlorophyll extraction. Can be fixed by discovering a more efficient method.
  2. I didn’t allow the leaves to boil long enough and need to be more patient.
  3. My light source (a flashlight) was either not powerful enough, or did not give out light in the right part of the electro-magnetic spectrum.
  4. My original hypothesis, that extracted chlorophyll will glow when exposed to a light source, is wrong.
  5. Yet to be discovered.

A cursory search on google and I discovered that my original hypothesis appears to be correct, but my methods are lacking. At least I know I’m on the right track.

I’m purposely avoiding looking at how other people have done this in the past. I want to work it out on my own. Who knows what I might figure out along the way?

First, I’m going to try to find a better method of chlorophyll extraction. Alcohol might be a better choice than water, or perhaps an acid or base. I’ll also try boiling leaves in water for a longer period of time.

Second, I’ll find different light sources, perhaps a UV light source, like a black light, and an IR light source (a remote control perhaps?).

I think I’ll also try to locate some thicker leaves than the ones I’m using. Perhaps elm’s just aren’t a good source.

If I’m only changing one variable at a time, I’ll be busy for a while!